Summary: A new study reveals how genetic deficits, particularly in the Tbx1 gene, are linked to reduced brain volume and altered social behavior, findings relevant to autism and schizophrenia. Researchers observed smaller amygdala volumes in Tbx1-deficient mice, a brain region key to emotional regulation and social behavior. These mice displayed less interest in social interaction, relying more on non-social cues like bedding preferences.
The study highlights the role of genetic variants in shaping brain development and behavior. Future research aims to explore critical developmental periods for potential therapeutic interventions targeting amygdala shrinkage. This work opens new avenues for understanding psychiatric disorders and their underlying genetic mechanisms.
Key Facts:
- Tbx1-deficient mice show reduced amygdala volume, affecting social behavior.
- Social ambivalence in mice correlates with alterations in brain regions like the amygdalo-piriform transition area.
- Amygdala shrinkage appears to originate in embryonic development, suggesting early intervention potential.
Source: UT San Antonio
A study published in Molecular Psychiatry found that brain volume changes correlate with social behavior differences in psychiatric conditions such as autism spectrum disorder and schizophrenia.
The study, led by Noboru Hiroi, PhD., professor in the Department of Pharmacology, Joe R. and Teresa Lozano Long School of Medicine at The University of Texas Health Science Center at San Antonio (UT Health San Antonio), showed that a deficit in a specific gene was associated with social behavioral differences in mice; differences that are often present in psychiatric disorders.
Genetic variants linked to some psychiatric conditions
Copy number variants (CNVs) are genetic alterations where segments of chromosomes exhibit deviations from the usual number of copies. These genomic variations are increasingly recognized for their influence on brain structure and their association with psychiatric disorders.
CNVs often encompass multiple genes, yet the specific contributions of individual genes within these variants to brain development and psychiatric conditions remain largely unexplored.
One notable example is the 22q11.2 region of human chromosome 22, which harbors more than 30 protein-coding genes. Among these, the Tbx1 gene has emerged as a critical regulator of stem cell function in the brain, as highlighted in prior studies by Hiroi and colleagues.
Variants in Tbx1 are strongly associated with a range of neurodevelopmental and psychiatric disorders, including autism spectrum disorders, schizophrenia, intellectual disability and developmental delay.
Understanding the role of Tbx1 and other genes within this region is essential for elucidating the mechanisms underlying CNV-associated brain abnormalities and psychiatric outcomes.
“Dr. Hiroi’s pioneering research in the Department of Pharmacology has significantly advanced our understanding of the genetic underpinnings of psychiatric disorders including autism spectrum disorder and schizophrenia,” said Daniel Lodge, PhD, professor and chair of the Department of Pharmacology at the Long School of Medicine at UT Health San Antonio.
“His recent study on Tbx1 not only highlights the complexity of gene-phenotype interactions but also illustrates how precise volumetric analyses can unravel the neural correlates of behavioral impairments, paving the way for potential therapeutic interventions.”
Brain volume differences
For this study, the research team created mice without the Tbx1 gene, then used volumetric MRI analysis to explore brain volume differences in these mice compared to wild-type mice. They also evaluated social behavior of mice with Tbx1 deletion compared to unaltered mice.
Hiroi discovered that Tbx1-deficient mice had decreased volume in their amygdala and surrounding cortical regions and, most notably, in a small, enigmatic subregion of the amygdala called the amygdalo-piriform transition area.
The amygdala is a critical region for emotional behavior regulation and, while not well-understood, the amygdalo-piriform transition area is connected to many brain regions that are involved in the processing of sensory and emotional cues.
Desire for social interaction affected
Animals and humans learn to use cues and context to decide if there is positive value in social experiences, Hiroi said. As social creatures, mice tend to choose interaction when given a choice to isolate or gather with other mice.
In this study, wild-type mice and Tbx1-deficient mice were given a choice of two enclosures – one with another mouse and one without. While wild-type mice always chose the enclosure with another mouse, Tbx1-deficient mice were ambivalent as to whether or not there was another mouse present and chose a location based on other cues, such as the location with a preferred type of bedding.
“This study suggests that Tbx1 deficiency might contribute to humans’ capacity to evaluate positive incentive values of social experiences,” Hiroi said.
What comes next?
Hiroi is taking the work a step further by teaming with Jason Pugh, PhD, in the UT Health San Antonio Department of Cellular and Integrative Physiology, to examine excitability of a specific type of neuron in the amygdalo-piriform transition area of Tbx1-deficient mice.
They are also working on models that could initiate and restore Tbx1 heterozygosity (having two versions of the same gene) at any stage of development. When complete, Hiroi said they plan to use this model to discover the critical developmental period for amygdala shrinkage.
Amygdala volume reduction appears to originate in the embryonic phase, Hiroi said, so therapeutic intervention could target this point. Additionally, the degree of amygdala shrinkage could be a biomarker of impaired appreciation of social experiences for people with autism spectrum disorder or schizophrenia.
“This study can serve as a catalyst to translationally link basic science with human brain imaging studies and studies of patients with neurodevelopmental disorders, which are already major strengths of UT Health San Antonio,” said Hiroi.
Other authors of Hiroi’s Tbx1 study also are with the Department of Pharmacology at UT Health San Antonio, as well as with the Institute of Development, Aging and Cancer of Tohoku University, Sendai, Japan; the National Institutes for Quantum and Radiological Science and Technology, Chiba, Japan; and the Laboratory of Human-Animal Interaction and Reciprocity at Azabu University, Sagamihara, Kanagawa, Japan.
About this genetics, autism, and schizophrenia research news
Author: Steven Lee
Source: UT San Antonio
Contact: Steven Lee – UT San Antonio
Image: The image is credited to Neuroscience News
Original Research: Open access.
“Highly demarcated structural alterations in the brain and impaired social incentive learning in Tbx1 heterozygous mice” by Noboru Hiroi et al. Molecular Psychiatry
Abstract
Highly demarcated structural alterations in the brain and impaired social incentive learning in Tbx1 heterozygous mice
Copy number variants (CNVs) are robustly associated with psychiatric disorders and changes in brain structures. However, because CNVs contain many genes, the precise gene-phenotype relationship remains unclear.
Although various volumetric alterations in the brains of 22q11.2 CNV carriers have been identified in humans and mouse models, it is unknown how each gene encoded in the 22q11.2 region contributes to structural alterations, associated mental illnesses, and their dimensions.
Our previous studies identified Tbx1, a T-box family transcription factor encoded in the 22q11.2 CNV, as a driver gene for social interaction and communication, spatial and working memory, and cognitive flexibility.
However, it remains unclear how TBX1 impacts the volumes of various brain regions and their functionally linked behavioral dimensions. In this study, we used volumetric magnetic resonance imaging analysis to comprehensively evaluate brain region volumes and behavioral alterations relevant to affected structures in congenic Tbx1 heterozygous mice.
Our data showed that the volumes of the anterior and posterior portions of the amygdaloid complex and its surrounding cortical regions were most robustly reduced in Tbx1 heterozygous mice. In an amygdala-dependent task, Tbx1 heterozygous mice were impaired in their ability to learn the incentive value of a social partner.
The volumes of the primary and secondary auditory cortexes were increased, and acoustic, but not non-acoustic, sensorimotor gating was impaired in Tbx1 heterozygous mice.
Our findings identify the brain’s regional volume alterations and their relevant behavioral dimensions associated with Tbx1 heterozygosity.
