Summary: Researchers discovered that gut bacteria produce a hormone involved in pregnancy and postpartum depression treatment by modifying steroids in bile. This research highlights the microbiome’s role as an endocrine organ influencing human health.
Findings suggest gut bacteria could be targeted to treat mental health conditions. The study opens new avenues for understanding gut-brain interactions.
Key Facts:
- Hormone Production: Gut bacteria produce allopregnanolone, a hormone linked to pregnancy and mood regulation.
- Microbiome’s Role: The microbiome acts as an endocrine organ, influencing human health and behavior.
- Potential Therapies: Research suggests targeting gut bacteria could help treat mental health conditions like postpartum depression.
Source: Harvard
It turns out flatulence can serve a purpose beyond being uncomfortable or funny: Gas released by some gut bacteria stimulates other gut bacteria to produce a hormone involved in pregnancy and in an FDA-approved treatment for postpartum depression, according to new research led by Harvard Medical School scientists.
The work shows how gut bacteria can produce new hormones from steroids in bile and, in doing so, act like an endocrine organ. This research adds to the growing list of ways gut microbiota may influence human biology and health.
The study also provides new evidence that doctors could one day treat or prevent certain kinds of mental health conditions by manipulating the gut microbiome.
The findings are published May 24 in Cell.
“While it’s common knowledge that gut health is important to our overall well-being, exactly how bacteria that reside in our GI tract interact with one another and with our own cells to impact our mental health is still being uncovered,” said first author Megan McCurry, who conducted the work as a graduate student and postdoctoral research fellow in the lab of Sloan Devlin in the Blavatnik Institute at HMS.
“This work reveals how certain gut bacteria perform a chemical transformation that produces a steroid that could impact women’s health and postpartum depression.”
Bugs making drugs
Working in lab dishes and with mice, McCurry and colleagues found two related kinds of bacteria in the human gastrointestinal tract — Gordonibacter pamelaeae and Eggerthella lenta — that can modify steroids. They further found that this chemical modification occurs in the opposite direction from how human cells make steroids.
“Our cells make steroids only in the oxidative direction, that is, losing electrons, whereas we’ve shown that gut bacteria can go in the reverse direction, known as reduction, or gaining electrons — making the bacterial transformation unique,” said Devlin.
The team found that the bacteria can alter corticoids found in bile — steroids that play roles in immune function and metabolism. The bacteria turn them into progesterone derivatives, which are sex hormones and neurosteroids that affect the brain and nervous system.
“We know that the human body makes progesterone in the adrenal glands, placenta, and ovaries. Our work suggests that the microbiome acts as an additional endocrine organ,” said Devlin, HMS associate professor of biological chemistry and molecular pharmacology and senior author of the study.
Devlin and team found that one of the progesterone derivatives that gut bacteria produce is allopregnanolone, so named a century ago because the body makes it during pregnancy.
In partnership with Andrea Edlow, HMS associate professor of obstetrics, gynecology, and reproductive biology at Massachusetts General Hospital, the team investigated allopregnanolone production in pregnancy.
Analyzing the fecal samples of study participants revealed that women in their third trimester of pregnancy not only had 100 times higher levels of allopregnanolone but also had more genetic traces of the two gut bacteria than people who were not pregnant.
The findings suggest that gut bacteria contribute to allopregnanolone production during pregnancy.
Researchers have linked low levels of allopregnanolone to postpartum depression and other mood and psychiatric disorders, and allopregnanolone is used as an FDA-approved drug known as brexanolone to treat postpartum depression.
With that in mind, Devlin and colleagues want to expand their fecal sample study to track participants across the first, second, and third trimesters and after delivery and see who develops postpartum depression.
The goal is to better understand whether gut bacteria contribute to allopregnanolone levels throughout pregnancy and drive risk for or protect against postpartum depression.
If the researchers find an effect, “down the road, as a community, we could think about microbiome-targeted therapies for neurological conditions like depression,” said Devlin.
Behind the chemistry
The researchers also revealed how the two bacteria do their chemical transformations.
First, they identified the genes at work. Second, they found that the G. pamelaeae and E. lenta bacteria need hydrogen — one of the gases other bacteria in our GI tract produce as they digest food.
“The effects of gas on bacterial metabolism have been largely overlooked, likely because studying it in the lab is very difficult,” she said. “Our work suggests that there are likely to be other gut bacterial processes that are significantly affected by, basically, flatulence.”
The team hopes the work demonstrates that the chemistry conducted by gut bacteria has an impact on human health and behavior, in this case during pregnancy.
“Prior to our work, the prevailing understanding was that the host makes steroids; the microbiome was not a part of the conversation,” said Devlin.
“We hope this work convinces people that gut bacteria modify steroids to produce molecules that can affect host functions, including mood and behavior.”
Funding:
McCurry is now a scientist at the biotech company Holobiome. Additional authors are Gabriel D’Agostino and Jasmine Walsh, both chemical biology PhD students in the Harvard Kenneth C. Griffin Graduate School of Arts and Sciences studying in the Devlin lab, as well as Jordan E. Bisanz, Ines Zalosnik, Xueyang Dong, David J. Morris, Joshua R. Korzenik, Emily Balskus, Peter J. Turnbaugh, and Jun R. Huh, associate professor of immunology in the Blavatnik Institute at HMS.
The study was funded by the National Institutes of Health (grants R35GM128618, R01DK110559, R01HL122593, R01HD100022), an Alfred P. Sloan Fellowship, the Simons Foundation (SFARI grant 870754), National Science Foundation (Alan T. Waterman Award CHE-20380529), and the Resnek Family Center for Primary Sclerosing Cholangitis Research.
McCurry was funded through an NSF Graduate Research Fellowship (no. DGE1745303), an HMS Christopher T. Walsh Graduate Student Fellowship award, an HMS Graduate Program in Bacteriology NIH T32 grant, and an HMS BCMP Van Maanen Fellowship award. Balskus is a Howard Hughes Medical Institute Investigator. Proteomic analyses were performed at the HMS BCMP MassSpec Center, and SEM imaging, consultation, and services were performed in the HMS Electron Microscopy Core Facility.
Devlin is an ad hoc consultant for Axial Therapeutics. McCurry and Devlin are co-inventors on a provisional patent related to this work. Huh serves as a consultant for CJ CheilJedang and is a co-founder and consultant for Interon Laboratories. He received research funding from CJ Bioscience outside the submitted work. Edlow serves as a consultant for Mirvie, Inc., outside the submitted work and receives research funding from Merck & Co., Inc., outside the submitted work. All other authors declare no competing interests.
About this microbiome, pregnancy, and PPD research news
Author: STEPHANIE DUTCHEN
Source: Harvard
Contact: STEPHANIE DUTCHEN – Harvard
Image: The image is credited to Neuroscience News
Original Research: Closed access.
“Gut bacteria convert glucocorticoids into progestins in the presence of hydrogen gas” by Megan McCurry et al. Cell
Abstract
Gut bacteria convert glucocorticoids into progestins in the presence of hydrogen gas
Highlights
- Gordonibacter and Eggerthella 21-dehydroxylate host corticoids to produce progestins
- Bacterial production of hydrogen gas promotes 21-dehydroxylation
- The 21-dehydroxylation genes were identified using comparative and functional genomics
- Progestin levels and gene cluster abundance were significantly higher during pregnancy
Summary
Recent studies suggest that human-associated bacteria interact with host-produced steroids, but the mechanisms and physiological impact of such interactions remain unclear.
Here, we show that the human gut bacteria Gordonibacter pamelaeae and Eggerthella lenta convert abundant biliary corticoids into progestins through 21-dehydroxylation, thereby transforming a class of immuno- and metabo-regulatory steroids into a class of sex hormones and neurosteroids.
Using comparative genomics, homologous expression, and heterologous expression, we identify a bacterial gene cluster that performs 21-dehydroxylation.
We also uncover an unexpected role for hydrogen gas production by gut commensals in promoting 21-dehydroxylation, suggesting that hydrogen modulates secondary metabolism in the gut.
Levels of certain bacterial progestins, including allopregnanolone, better known as brexanolone, an FDA-approved drug for postpartum depression, are substantially increased in feces from pregnant humans.
Thus, bacterial conversion of corticoids into progestins may affect host physiology, particularly in the context of pregnancy and women’s health.
