Summary: Groundbreaking research demonstrates a significant advancement in heart transplant preservation, enabling pig hearts to be kept alive for over 24 hours using normothermic ex-vivo heart perfusion (NEHP). This method, which maintains donor hearts in a near-physiological state, could revolutionize the transplantation field by extending the viability period far beyond the current six-hour window.
By incorporating techniques like hemofiltration, plasma exchange, and intermittent left atrial perfusion, the study opens possibilities for increasing the donor pool and improving logistics in heart transplantation. This pioneering work holds promise for clinical application, aiming to make more hearts available for those in need.
Key Facts:
- NEHP technique successfully preserved pig hearts for more than 24 hours, a potential breakthrough for extending the preservation time of donor hearts.
- The study introduces modifications to standard NEHP, such as hemofiltration and plasma exchange, significantly improving heart preservation.
- This research could increase the donor heart pool by overcoming logistical limitations and allowing for a better assessment of donor heart viability.
Source: Frontiers
56 years after the first human-to-human heart transplantation, more than 5,000 hearts are transplanted each year around the world. This number is far from enough to give a new heart to everyone who needs one, with up to 50,000 people needing one at any time.
Depending on how ill they are and physiological and logistical factors, candidates may wait for years. A bottleneck is the availability of suitable donor hearts – partly due to the very short time window for transplantation once the heart is removed from the deceased donor.
The current ‘gold standard’ for preserving donor hearts is cold static storage (CSS), where hearts are kept on ice until transplantation.
Transplantation is most successful when CSS lasts less than six hours, before the heart or its blood vessels undergo damage. Periods up to 12 hours are sometimes possible, but require mechanical life support such as extracorporeal membrane oxygenation (ECMO) for several days on the recipient.
Prolonging the storage period beyond six hours without the need for ECMO would thus be a medical breakthrough.
Now, researchers publishing in Frontiers in Cardiovascular Medicine have shown it’s possible to keep transplanted pig hearts alive outside the body for more than 24 hours using a process called normothermic ex-vivo heart perfusion (NEHP).
“If translated to humans, this would be a major improvement to the six-hour-long time window in standard clinical practice,” said Dr Robert Bartlett, an emeritus professor and head of the Extracorporeal Life Support Laboratory at the University of Michigan Medical School at Ann Arbor.
Keep it pumping
NEHP means that hearts, once removed from their donor, are kept in a partly physiological state at room temperature by pumping oxygenated, nutrient-rich fluid (‘perfusate’) derived from blood plasma through them until transplantation.
Drugs and tissue-repairing stem cells can be delivered to the heart through the perfusate. Currently, the only variant of NEHP approved for clinical use by the US Food and Drug Administration is Transmedics-OCS, which like CSS is limited to six hours.
Over the past seven years, the Extracorporeal Life Support Laboratory has worked on steadily extending the shelf-life of donor hearts through improvements to NEHP.
Their previous experiments have shown that a critical step is to filter the perfusate to remove all molecules smaller than 26 kilodalton. Without this, for unknown reasons, hearts quickly become unusable for transplantation.
Here, Bartlett and his colleagues kept the hearts of 30 immature and 10 juvenile pigs alive for various periods with experimental variants of NEHP. For example, the perfusate for all donor hearts was a solution of blood plasma and packed red blood cells (from additional healthy pigs), electrolytes, glucose, and antibiotics.
The perfusate was pumped through the heart at a mean rate of 0.7 milliliter per minute per gram heart weight, and replaced every 60 minutes.
HEHP – with a difference
They then compared the effects between three variants: NEHP with hemofiltration to continuously purify the perfusate and remove toxins (10 immature pig hearts); NEHP where the plasma component in the perfusate was exchanged continuously (five immature hearts); and control NEHP without modifications (15 immature hearts).
To test these methods on larger hearts, they also used NEPH with hemofiltration on five hearts from juvenile pigs, and NEPH with an additional modification (intermittent left atrial perfusion or iLA) on a further seven hearts from juveniles, to monitor heart function. In iLA, a fixed volume of blood is injected into the left atrium at regular intervals, to test its continued power to eject this blood.
The authors monitored the health of the preserved hearts in real time by visually checking its contractility, rhythm, color, and edema, and by measuring the concentration of lactate (a by-produce of cellular damage) every hour.
Each heart was maintained until it went into asystole or arrhythmia, showed minimal systolic blood pressure in the left ventricle, or showed elevated lactate concentrations for at least two hours.
Strong improvements
All control hearts died between 10 and 24 hours after removal from the donor, while all hearts that had been maintained with modifications to standard NEPH survived for the full 24 hours.
The authors conclude that hemofiltration, plasma exchange, and iLA are major improvements that enable the routine preservation of hearts to beyond one day. Which of the latter three methods is better can’t be answered yet.
“I think the major difference will be when we extend our experiments beyond 24 hours, where perhaps plasma exchange is better as larger toxins can be removed. iLA also seems a major improvement, as in principle it would allow NEPH to be used on hearts that have suffered injuries or have borderline function in the donor,” said Bartlett.
More hearts available for transplantation
“This work could ultimately increase the donor pool. First, by extending the preservation time, thus overcoming limitations due to logistics. Second, by giving an objective assessment of the viability of each potential donor heart, to reduce the number that currently aren’t used when it’s not clear how well they function inside the donor,” said Dr Alvaro Rojas-Pena, a research investigator at the same institute and the corresponding author of the study.
“The major challenge for clinical application will be the validation of the methods in humans. To this end, we have started to work with human hearts rejected for transplantation,” said Rojas-Pena.
About this neurology research news
Author: Mischa Dijkstra
Source: Frontiers
Contact: Mischa Dijkstra – Frontiers
Image: The image is credited to Neuroscience News
Original Research: Open access.
“Extending heart preservation to 24 hours with normothermic perfusion” by Alvaro Rojas-Pena et al. Frontiers in Cardiovascular Medicine
Abstract
Extending heart preservation to 24 hours with normothermic perfusion
Cold static storage (CSS) for up to 6 h is the gold standard in heart preservation. Although some hearts stored over 6 h have been transplanted, longer CSS times have increased posttransplant morbimortality.
Transmedics® Organ Care System (OCS™) is the only FDA-approved commercial system that provides an alternative to CSS using normothermic ex situ heart perfusion (NEHP) in resting mode with aortic perfusion (Langendorff method). However, it is also limited to 6 h and lacks an objective assessment of cardiac function.
Developing a system that can perfuse hearts under NEHP conditions for >24 h can facilitate organ rehabilitation, expansion of the donor pool, and objective functional evaluation. The Extracorporeal Life Support Laboratory at the University of Michigan has worked to prolong NEHP to >24 h with an objective assessment of heart viability during NEHP. An NEHP system was developed for aortic (Langendorff) perfusion using a blood-derived perfusate (leukocyte/thrombocyte-depleted blood).
Porcine hearts (n = 42) of different sizes (6–55 kg) were divided into five groups and studied during 24 h NEHP with various interventions in three piglets (small-size) heart groups: (1) Control NEHP without interventions (n = 15); (2) NEHP + plasma exchange (n = 5); (3) NEHP + hemofiltration (n = 10) and two adult-size (juvenile pigs) heart groups (to demonstrate the support of larger hearts); (4) NEHP + hemofiltration (n = 5); and (5) NEHP with intermittent left atrial (iLA) perfusion (n = 7). All hearts with NEHP + interventions (n = 27) were successfully perfused for 24 h, whereas 14 (93.3%) control hearts failed between 10 and 21 h, and 1 control heart (6.6%) lasted 24 h.
Hearts in the piglet hemofiltration and plasma exchange groups performed better than those in the control group. The larger hearts in the iLA perfusion group (n = 7) allowed for real-time heart functional assessment and remained stable throughout the 24 h of NEHP.
These results demonstrate that heart preservation for 24 h is feasible with our NEHP perfusion technique. Increasing the preservation period beyond 24 h, infection control, and nutritional support all need optimization.
This proves the concept that NEHP has the potential to increase the organ pool by (1) considering previously discarded hearts; (2) performing an objective assessment of heart function; (3) increasing the donor/recipient distance; and (4) developing heart-specific perfusion therapies.
